Additional file 1: of Elusive sources of variability of dystrophin rescue by exon skipping
Year: 2015
Type: article
Abstract: Proteolysis in dystrophin immunoblotting and band quantification. A) Dystrophin immunoblotting of six muscles (triceps, quadriceps, diaphragm, gastrocnemius, tibialis anterior, and heart). The panel shows the entire blot area of the cropped images shown in Fig. 2a. Mouse ear-tag IDs are shown on the gastrocnemius blot. B) For dystrophin band quantification, x-ray films were scanned, and densitometry analysis was carried out using a Bio-Rad GS-800 calibrated densitometer and Quantity One software. Band area to be quantified was determined by delineating the largest band, which was kept constant for quantification of all other lanes. Degradation products were not included in the quantification as shown. (PDF 128 kb)
Source: Figshare
Authors: Vila, Maria, Klimek, Margaret, Novak, James, Rayavarapu, Sree, Uaesoontrachoon, Kitipong, Boehler, Jessica, Fiorillo, Alyson, Hogarth, Marshall, Zhang, Aiping, Shaughnessy, Conner, Gordish-Dressman, Heather, Burki, Umar, Straub, Volker, Lu, Qi, Partridge, Terence, Brown, Kristy, Hathout, Yetrib, van den Anker, John, Hoffman, Eric, Nagaraju, Kanneboyina
Language: English
Subfield: Molecular Biology
Domain: Life Sciences
Open Access status: green
OpenAlex ID: W7137264980