HaDeX2: multi-dimensional analysis of hydrogen–deuterium exchange mass spectrometry data
Year: 2026
Type: article
Abstract: SUMMARY: Hydrogen-Deuterium Exchange Mass Spectrometry (HDX-MS) monitors deuterium uptake at the peptide level, in a time-dependent manner. It produces complex, multi-dimensional data that must be interpreted at minimum both the temporal and sequence levels. Specialized tools are therefore essential to preprocess, integrate, and analyze HDX-MS data and translate it into meaningful biological insights. HaDeX2 provides statistical inferences and their visualizations across five dimensions of HDX-MS data: protein sequence, time, biological states, peptide charge and experimental replicates. AVAILABILITY AND IMPLEMENTATION: HaDeX2 is freely available as an R package (https://github.com/hadexversum/HaDeX2; https://doi.org/10.5281/zenodo.18543703) and web server (https://hadex2.mslab-ibb.pl/). To run the GUI locally, users should install a dedicated companion package (https://github.com/hadexversum/HaDeXGUI).
Source: Bioinformatics
Institutions: Institute of Biochemistry and Biophysics, Polish Academy of Sciences, Polish Academy of Sciences, Medical University of Białystok, University of Wrocław, Warsaw University of Technology, Rega Institute for Medical Research, KU Leuven, Centre National de la Recherche Scientifique, Institut de Pharmacologie et de Biologie Structurale, Université de Toulouse, University of Bradford, Vilnius University
Language: English
Subfield: Spectroscopy
Field: Chemistry
Domain: Physical Sciences
Open Access status: gold
Estimated APC paid: $3,989
Awards: G1319244215, G1881005258, G4644740001
OpenAlex ID: W7137102374