Additional file 6: Figure S5. of WNT4 mediates estrogen receptor signaling and endocrine resistance in invasive lobular carcinoma cell lines
Year: 2016
Type: article
Abstract: Establishment of long-term estrogen-deprived variants of ILC cell lines. a Schematic representation of development of LTED lines together with representative phase-contrast images (original magnification × 100) of resulting lines. b Once established (i.e., able to be passaged routinely in T75 flasks), lysates were collected from LTED lines, parental ILC cells, and MCF-7 cells. Immunoblotting was performed as described in the Methods section. c Endocrine response was assessed in ILC-LTED vs hormone-deprived parental cells. BCCL cells were treated as indicated, and proliferation was assessed 6 days after treatment. Proliferation is shown as baseline subtracted vs vehicle (0.01 % EtOH) treatment. *p < 0.05 treatment vs vehicle control (t test). +Immediately after establishment, 134:L/B maintained modest endocrine responsiveness, but within several passages this was lost. Repeat experiments revealed no difference between treatment and vehicle. IMEM Improved minimal essential medium. (PDF 198 kb)
Source: Figshare
Subfield: Genetics
Domain: Life Sciences
Open Access status: green
OpenAlex ID: W7137139452
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