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MOESM3 of Use of aminoglycoside 3â ˛ adenyltransferase as a selection marker for Chlamydia trachomatis intron-mutagenesis and in vivo intron stability

Year: 2015

Type: article

Abstract: Additional file 3: Figure S3. Sequence map of the rsbV1::GII(bla) locus. The rsbV1::GII(bla) locus was PCR amplified from both DFCT13 and DFCT16 and cloned into pJET for Sanger sequencing. The intron inserts into the rsbV1 open reading frame after position 28 (positon 1 is the A in ATG) resulting in a protein sequence differing from the wild type RsbV1 at amino acid 11 and a stop codon at position 96. The the intron sequence is highlighted in pink. The entire intron sequence is not shown (the entire mobile intron sequence is shown in pink in Figure S1D).

Source: Figshare

DOI: 10.6084/m9.figshare.4452167

Authors: Lowden, Nicole, Yeruva, Laxmi, Johnson, Cayla, Bowlin, Anne, Fisher, Derek

Language: English

Topic: Bacterial Genetics and Biotechnology

Subfield: Genetics

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

SDG: Good health and well-being

Open Access status: green

OpenAlex ID: W7137362154

FWCI:

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