MOESM1 of Enhancing poly(3-hydroxyalkanoate) production in Escherichia coli by the removal of the regulatory gene arcA
Year: 2016
Type: article
Abstract: Additional file 1: Figure S1A. GPC chromatogram of purified poly(3-hydroxybutyrate) (C4) produced by RSC02, plotted as normalized intensity vs retention time. No data available for LSBJ. Figure S1B, C GPC chromatogram of purified poly(3-hydroxyvalerate) (C5) produced by LSBJ (B) and RSC02 (C), plotted as normalized intensity vs retention time. Figure S1D, E GPC chromatogram of purified poly(3-hydroxyhexanoate) (C6) produced by LSBJ (D) and RSC02 (E), plotted as normalized intensity vs retention time. Figure S1F, G GPC chromatogram of purified poly(3-hydroxyheptanoate) (C7) produced by LSBJ (F) and RSC02 (G), plotted as normalized intensity vs retention time. Figure S1H, I GPC chromatogram of purified poly(3-hydroxyoctanoate) (C8) produced by LSBJ (H) and RSC02 (I), plotted as normalized intensity vs retention time. Figure S1J, K GPC chromatogram of purified poly(3-hydroxydecanoate) (C10) produced by LSBJ (J) and RSC02 (K), plotted as normalized intensity vs retention time. Figure S1L, M GPC chromatogram of purified poly(3-hydroxydodecanoate) (C12) produced by LSBJ (L) and RSC02 (M), plotted as normalized intensity vs retention time. Figure S2A 1H-NMR (600 MHz, CDCl3); δ 5.28-5.23 (sext, 1H), 2.62-2.45 (m, 2H), 1.28-1.25 (d, 3H). Figure S2B 1H-NMR (600 MHz, CDCl3); δ 5.17-5.13 (p, 1H), 2.59-2.50 (m, 2H), 1.66-1.59 (m, 2H), 0.91-0.88 (t, 3H). The asterisk (*) at δ 1.53 denotes a water impurity (Fulmer et al. 2010). Figure S2C 1H-NMR (600 MHz, CDCl3); δ 5.21-5.18 (p, 1H), 2.59-2.48 (m, 2H), 1.60-1.54 (m, 2H), 1.35-1.27 (m, 2H), 0.91 (t, 3H). The asterisk (*) at δ 1.53 denotes a water impurity (Fulmer et al. 2010). Table S1: Poly(3-hydroxydecanoate) yield comparison between LSBJ, RSC02, RSC04.
Source: Figshare
Language: English
Subfield: Biomaterials
Field: Materials Science
Domain: Physical Sciences
Open Access status: green
OpenAlex ID: W7137889335