MOESM3 of Regulation of chaperone binding and nucleosome dynamics by key residues within the globular domain of histone H3
Year: 2016
Type: article
Abstract: Additional file 3: Fig. S3. Histone mutations do not alter Spt2, Spt6, and Spt16, occupancy over lowly transcribed genes. (a) ChIP analysis was performed on chromatin prepared from strains expressing HHTS-HHFS alleles (YS454-YS456, YS493-YS495) or the indicated histone mutant alleles (YS458-YS462, YS465, YS471, YS472, YS474, YS504-YS506, YS518, YS519, YS521, YS525-YS527) that were grown in YPD at 30 °C. The amount of immunoprecipitated DNA was determined by qPCR and is shown as a percentage of the input material and represents the mean ± SEM of three biological replicate experiments. Factor occupancy was measured within the coding region of a GAL1. (b) Factor occupancy at CYC1, a lowly transcribed gene, was determined as described in a.
Source: Figshare
Authors: Hainer, Sarah, Martens, Joseph
Language: English
Subfield: Molecular Biology
Domain: Life Sciences
Open Access status: green
OpenAlex ID: W7138333295