Additional file 1: Figure S1. of Inhibition of fatty acid desaturation is detrimental to cancer cell survival in metabolically compromised environments
Year: 2016
Type: article
Abstract: Cancer cell lines used in this study, effect of shRNA mediated silencing and expression of SCD in breast cancer. (a) Information regarding cancer cell line type, cancer type, site of origin and hormone receptor status. (b) DU145 cells were transduced with TetOnPLKO plasmids expressing inducible shRNAs targeting SCD (shSCD #1 and shSCD #2) or non-targeting control (shNTC). Cells were treated with 0.5Îźg/ml doxycycline (DOX) or solvent (ethanol, EtOH) for 6 days in medium supplemented with 1% FCS (LS). Levels of SCD mRNA were determined using RT-qPCR. (c) Cells were seeded at low density and treated with 0.5Âľg/ml doxycycline (DOX) or solvent (EtOH) for 12 days. Cell density was determined by crystal violet staining. Representative images are shown left and colorimetric quantitation of three independent biological replicates is shown on right. (d) Differential SCD expression in different breast cancer subtypes (Curtis dataset, Oncomine). (e) Correlation of SCD expression with tumour grade in four publicly available breast cancer datasets (Oncomine). (f) Differential SCD expression in IDC patient samples divided according to survival at 1, 3 and 5 years post diagnosis (TCGA dataset, Oncomine). (PDF 876 kb)
Source: Figshare
Authors: Peck, Barrie, Schug, Zachary, Zhang, Qifeng, Dankworth, Beatrice, Jones, Dylan, Smethurst, Elizabeth, Patel, Rachana, Mason, Susan, Jiang, Ming, Saunders, Rebecca, Howell, Michael, Mitter, Richard, Spencer-Dene, Bradley, Stamp, Gordon, McGarry, Lynn, James, Daniel, Shanks, Emma, Aboagye, Eric, Critchlow, Susan, Leung, Hing, Harris, Adrian, Wakelam, Michael, Gottlieb, Eyal, Schulze, Almut
Language: English
Subfield: Cancer Research
Domain: Life Sciences
Open Access status: green
OpenAlex ID: W7138091945