Year: 2026
Type: article
Abstract: The use of monoclonal fragments antigen binding (Fabs) is a prevalent methodology facilitating protein structure determination via both crystallography and cryo-EM. The development of a synthetic Fab against the BRIL domain improved the accessibility of this approach, providing a general fiducial applicable to any protein of interest via the simple curation of a BRIL fusion protein. Here, we document the generation of a T7 Express Δ cybC strain allowing contaminant-free bacterial expression of the synthetic anti-BRIL Fab BAG2. We also report the crystal structure of BAG2 in complex with native cytochrome b 562 , a complex arising from expression in canonical Escherichia coli strains.
Authors: Benjamin F. Cooper, Georgia L. Isom
Institution: University of Oxford
Language: English
Subfield: Materials Chemistry
Field: Materials Science
Domain: Physical Sciences
Open Access status: hybrid
Estimated APC paid: $3,520
OpenAlex ID: W7138124259
FWCI:
Cites:
Cited by:
Related to: