Work

Manipulation therapy alleviates neuropathic pain via the SOCS1 m 6 A epigenetic loop

Year: 2026

Type: article

Abstract: Background: Manipulation therapy (MT) relieves pain in nervous system disorders by reducing inflammation. Although evidence suggests MT is beneficial for neuropathic pain (NP), its molecular mechanisms remain unclear. This study investigated whether MT could reverse NP-related epigenetic changes and elucidated the potential mechanisms of NP. Methods: A) levels were assessed by colorimetric analysis, while target genes were validated using methylated RNA immunoprecipitation succeeded by quantitative polymerase chain reaction, immunofluorescence, Western blotting, and quantitative real-time polymerase chain reaction. Results: A methylation levels, consequently reducing the secretion of neuroinflammatory cytokines interleukin-1 beta (IL-1β), IL-6, and tumor necrosis factor-alpha (TNF-α), thereby alleviating NP. Conclusions: the SOCS1/TLR4 pathway.

Source: The Korean journal of pain

DOI: 10.3344/kjp.25124