Additional file 1: Text S1. of Engineering dynamic cell cycle control with synthetic small molecule-responsive RNA devices
Year: 2015
Type: article
Abstract: Plasmid construction. Figure S1. Overview of DNA staining and cell cycle quantification experiments. Figure S2. Representative histograms of cell count vs. DNA staining. Figure S3. Double thymidine block. Figure S4. siRNA knockdown of CDK4, CDK6, or both CDK4 and CDK6 in U2-OS cells. Figure S5. Ribozyme switch controls for arresting cells in G2/M. Figure S6. Ribozyme switch controls for arresting cells in G0/1. Figure S7. Plasmid maps. Table S1. Regulatory nodes tested in this study. Table S2. Ribozyme switch performance in yeast and HEK293. Table S3. Cell lines created in this study. Table S4. Primer sequences. Table S5. List of the plasmids used in this study. Table S6. Sequences of ribozyme controls and switches used in this study. (PDF 1609 kb)
Source: Figshare
Authors: Wei, Kathy, Smolke, Christina
Language: English
Subfield: Molecular Biology
Domain: Life Sciences
Open Access status: green
OpenAlex ID: W7138338232