Work

MOESM2 of Infection of rhesus macaques with a pool of simian immunodeficiency virus with the envelope genes from acute HIV-1 infections

Year: 2016

Type: article

Abstract: Additional file 2: Figure S2. Testing of virus production from SHIVKB9 vector system. To test the SHIVKB9 vector system, the pREC_nfl_SHIVKB9 and the complementary vector, pREC_cplt_R/U5/gag were co-transfected into 293T cells to produce SHIVKB9 virus in cell-free supernatant, which was then used to infect 174×CEM.CCR5 cells. SHIVKB9 virus production from 174×CEM.CCR5 cells is shown in (A) and is consistent with the previous reports of SHIVKB9 virus propagation by Reimann et al. [30]. SHIVKB9 virus was harvested from day 16 supernatant of the 174×CEM.CCR5 infection and used to infect U87.CD4.CCR5 cells (B).

Source: Figshare

DOI: 10.6084/m9.figshare.4329455

Authors: Krebs, Kendall, Tian, Meijuan, Asmal, Mohammed, Ling, Binhua, Nelson, Kenneth, Henry, Kenneth, Gibson, Richard, Li, Yuejin, Han, Weining, Shattock, Robin, Veazey, Ronald, Letvin, Norman, Arts, Eric, Gao, Yong

Language: English

Topic: HIV Research and Treatment

Subfield: Virology

Field: Immunology and Microbiology

Domain: Life Sciences

SDG: Good health and well-being

Open Access status: green

OpenAlex ID: W7138339230

FWCI:

Cites:

Cited by:

Related to: 10