Work

Additional file 1 of Stretching inhibits tumor growth in MMTV-PYMT via a direct mechanical effect

Year: 2026

Type: other

Abstract: Additional file 1. Procedure to characterize the tumor microenvironment. A. Met-1 tumor cells were injected into the loose subcutaneous tissue on the dorsal area of the third mammary fat pad. After 1 week of stretching, the tumors were dissected through the subcutaneous space, allowing the tumor to remain attached to the skin, and then the tissue was fixed in 10% neutral buffered formalin overnight. The tissue was placed face down on a glass bottom dish. The tumors and collagen matrix were imaged via multiphoton laser scanning microscopy and second harmonic imaging. The alignment of the collagen fibers was analyzed via CurveAlign software. B. Procedure to measure tumor volume using 3D ultrasound. Tumors were measured by 3D volume reconstruction by drawing around the tumor area to acquire a series of 2D 'slices' (1, 2, 3, 4…) that were assembled into a 3D dataset and processed using the VevoLab software (Fujifilm, VisualSonics). C Representative 3D ultrasound image of a No Stretch tumor. D Representative 3D ultrasound image of a Stretch tumor.

Source: Figshare

DOI: 10.6084/m9.figshare.31810199

Authors: Berrueta, Lisbeth, Bhan, Chandra, He, Yi, Thompson, Rebecca D., Safairad, Oscar, Doyle, Andrew D., Pérez, Paola, Martin, Daniel, Mezher, Mazen, Cartagena-Rivera, Alexander, Badger, Gary J., Langevin, Helene M.

Institutions: National Institute of Dental and Craniofacial Research, National Institute of Biomedical Imaging and Bioengineering, University of Vermont

Language: English

Topic: Cellular Mechanics and Interactions

Subfield: Cell Biology

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

SDG: Good health and well-being

Open Access status: green

OpenAlex ID: W7138921375

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