Heat-killed Streptococcus salivarius LAB813 Decreases Oral Cancer Cell Proliferation, Migration and Matrix Degradation
Year: 2025
Type: dissertation
Abstract: Oral squamous cell carcinoma (OSCC) is often diagnosed at advanced stages, resulting in poor prognosis and frequent recurrence. While probiotics have demonstrated anti-cancer potential mostly in gastrointestinal models, their roles in OSCC remains underexplored. This study investigated the impact of heat-killed Streptococcus salivarius LAB813 (HK813) on OSCC cell progression including proliferation, death, migration, and inflammation. HK813 maintained neutral pH and did not induce IL 6 or IL 8 expression in an OSCC cell line, UMSCC1. HK813 reduced UMSCC1 cell viability and proliferation in a time- and dose-dependent manner and significantly decreased migration and matrix degradation. The levels of MMP-9 levels, however, remained unchanged. Annexin V/PI staining indicated increased late apoptosis and necrosis, accompanied by modest activation of intrinsic and extrinsic caspases. These findings suggest that HK813 can suppress some key events in OSCC progression without triggering inflammation, highlighting its potential as a safe, non-viable probiotic candidate for oral cancer therapy.
Source: TSpace (University of Toronto)
Author: Liu, Anqi
Institution: University of Toronto
Language: English
Subfield: Food Science
Domain: Life Sciences
Open Access status: green
OpenAlex ID: W7139339372