Work

Generation of cynomolgus embryonic stem cell line (KP-CmESC-C1) from blastocysts produced by intracellular sperm injection

Year: 2026

Type: article

Abstract: Embryonic stem cells (ESCs) constitute a unique lab resource, as they can differentiate to all other cell types, making them pluripotent. We established cynomolgus ESCs using ICSI-derived blastocysts. Cynomolgus ESCs expressed pluripotent markers in immunostaining and fluorescence-activated cell sorting analyses. To investigate its differentiation potential, we confirmed the expression of layer markers from the three germ layers after embryonic body differentiation. Moreover, our ESC shows a normal genotype and no mycoplasma contamination.

Source: Stem Cell Research

DOI: 10.1016/j.scr.2026.103970

Authors: Jeongwoo Kwon, Yu-Jin Jo, Seung-Bin Yoon, Ji-Su Kim

Institution: Korea Research Institute of Bioscience and Biotechnology

Language: English

Topic: Pluripotent Stem Cells Research

Subfield: Molecular Biology

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

SDG: Good health and well-being

Open Access status: gold

Estimated APC paid: $2,360