Mg2+ Catalyzes Nonenzymatic RNA Primer Extension through a Concerted Outer-Sphere Mechanism
Year: 2026
Type: article
Abstract: The nonenzymatic replication of the primordial genetic material was an essential stage in the origin of life. One intensively studied model for this process is the template-directed extension of an RNA primer with imidazolium-bridged dinucleotide substrates. This reaction is catalyzed by divalent metal ions such as Mg2+, but the mechanism of catalysis remains poorly understood. We have utilized classical molecular dynamics together with hybrid quantum mechanics/molecular mechanics to investigate the catalytic role of Mg2+. We used these approaches to compute the free energy landscape along key reaction coordinates and to quantify the impact of Mg2+ coordination at each step of the primer extension reaction. The presence of Mg2+ in the reaction center significantly lowers the pKa of the nucleophilic 3′-OH group and reduces the probability of O2′ attack. Based on the energetics of potential reaction pathways, our results suggest a preferred mechanism in which Mg2+ becomes outer-sphere coordinated to the oxygen of the 3′-hydroxyl group of the extending primer followed by concerted proton transfer and nucleophilic attack on the phosphate of the incoming nucleotide. Our results demonstrate the dual structural and electronic roles of Mg2+ in catalysis and provide insights that may inform the search for metal ion chelators that further enhance nonenzymatic primer extension.
DOI: 10.1021/jacs.6c06167
Institutions: University of Chicago, Boston University
Language: English
Subfield: Astronomy and Astrophysics
Field: Physics and Astronomy
Domain: Physical Sciences
Open Access status: hybrid
Estimated APC paid: $4,500
OpenAlex ID: W7163882269