Year: 2026
Type: article
Abstract: Materials and Methods Selection of GABA-Producing L. lactis StrainsL. lactis strains were cultured in MRS broth supplemented with 1% glucose and 1% monosodium glutamate (MSG).GABA production in the culture supernatant was monitored daily.For GABA detection, 0.5 μl of each culture supernatant was spotted onto silica gel 60 F254 TLC plates (Merck, Germany), developed with a solvent system of acetic acid:butanol:water (3:5:2) containing 0.4% (w/v) ninhydrin, and visualized after drying in an oven.The novel strain Lactococcus lactis PL186 exhibits high GABA productivity in vitro and has demonstrated proven safety as a probiotic.In mice, this strain adheres to the intestinal mucosa, resulting in increased GABA levels in both serum and brain tissue.Additionally, L. lactis PL186 effectively colonizes the human gut and elevates GABA concentration in urine, highlighting its potential for systemic GABA enhancement.Oral administration of GABA-producing probiotics such as L. lactis PL186 therefore supports persistent endogenous GABA biosynthesis, helps maintain relatively stable systemic concentrations, and enables prolonged functional benefits.To our knowledge, this is the first report demonstrating that L. lactis can produce GABA that circulates within the human body.
Institution: Seoul Women's University
Language: English
Subfield: Food Science
Domain: Life Sciences
Open Access status: bronze
Awards: G1744436153, G2589381140
OpenAlex ID: W7140087033
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