Work

Molecularly Imprinted Polymers as Biomimetic Test Zones in Paper-Based Nucleic Acid Assays—Comparing Vertical and Lateral Flow Formats

Year: 2026

Type: article

Abstract: The development of rapid and sensitive point-of-care nucleic acid tests benefits from robust synthetic recognition elements. Here, a biotin-specific molecularly imprinted polymer (MIP) was synthesized using an optimized protocol and integrated as a biomimetic test zone into two paper-based formats: nucleic acid vertical flow (NAVF) and nucleic acid lateral flow (NALF). Both platforms were evaluated for the detection of double-tagged PCR amplicons from Escherichia coli. NAVF enabled a 3 min visual readout with an LOD of 1.00 × 10−2 ng mL−1. NALF provided a total assay time of <15 min and achieved a visual LOD of 3.17 × 10−2 ng mL−1. Overall, the results demonstrate the versatility of biotin-MIPs as stable synthetic receptors for rapid, low-cost paper-based nucleic acid assays, with NAVF prioritizing speed and design flexibility and NALF prioritizing higher analytical sensitivity.

Source: Biosensors

DOI: 10.3390/bios16030175

Authors: Jennifer Marfà, Anaixis del Valle, Maria Del Pilar Taboada Sotomayor, María Isabel Pividori

Institutions: Universitat Autònoma de Barcelona, Universidade Estadual Paulista (Unesp)

Language: English

Topic: Biosensors and Analytical Detection

Subfield: Biomedical Engineering

Field: Engineering

Domain: Physical Sciences

Open Access status: gold

Estimated APC paid: $2,654

Funders: Generalitat de Catalunya, Ministerio de Ciencia e Innovación, Ministerio de Universidades