Work

Fluorescent bioreporters for assessing nitrogenase expression and rhizobial nodule occupancy in Lotus japonicus and Pisum sativum

Year: 2026

Type: article

Abstract: ABSTRACT A bioreporter system based on sfYFP, sfCFP, and mScarlet-I in Mesorhizobium japonicum and sfYFP and sfCFP in Rhizobium leguminosarum , driven by nifH consensus promoters was established to monitor occupancy and nitrogen fixation events within legume host nodules. These bioreporter plasmids expand established rhizobium sfGFP system to new hosts and fluorophores.

Source: Microbiology Resource Announcements

DOI: 10.1128/mra.00103-26

Authors: Gayathri Senanayake, Amanda B. Pease, Chandan K. Gautam, Birgit M. Prüß, Barney A. Geddes

Institution: North Dakota State University

Language: English

Topic: Legume Nitrogen Fixing Symbiosis

Subfield: Plant Science

Field: Agricultural and Biological Sciences

Domain: Life Sciences

Open Access status: diamond

Funders: U.S. Department of Agriculture, North Dakota State University, Foundation for Food and Agriculture Research, National Institute of Food and Agriculture

Awards: G1653608509, G2349022801, G4082545328, G702641081