Data and code from: Microfluidic nanomagnetically isolated neuron- and astrocyte-derived extracellular vesicles to differentiate Lewy body and Alzheimer’s disease
Year: 2026
Type: dataset
Abstract: Identifying plasma-based biomarkers that can accurately differentiate Lewy body disease (LBD) from Alzheimer’s disease (AD) remains a major challenge. Extracellular vesicles (EVs), which carry molecular cargo from their parent cells and can cross the blood-brain barrier, offer a new path forward. We developed the multiplexed Track-Etch magnetic NanoPOre (mTENPO) platform, a highly parallelized microfluidic technology for cell-specific EV isolation, and demonstrated independent enrichment of GluR2+ (neuron-derived) and GLAST+ (astrocyte-derived) EVs from the antemortem plasma of 137 autopsy-confirmed LBD, AD, mixed pathology, and control subjects. By integrating miRNA sequencing of GluR2+ and GLAST+ EV cargo with plasma measurements of Aβ40, Aβ42, tau, p-Tau181, and p-Tau231, we identified a multimodal 15-feature panel that more comprehensively reflects brain pathology than conventional biomarkers. Using 10-fold cross-validation to mitigate overfitting, the panel achieved an accuracy of 0.95 and an area under the curve of 0.96 for distinguishing LBD versus AD.
Source: DRYAD
Authors: Yang, Stephanie, Lin, Andrew, Shen, Hanfei, Pappalardo, Laura, Spychalski, Griffin, Rosario, Jean, Forsberg, Leah, Grant, Kiera, Buser, Joshua, Savica, Rodolfo, O'Bryant, Sid, Jones, David, Dickson, Dennis, Reichard, R. Ross, Nguyen, Aivi, Meaney, David, Boeve, Bradley, Ross, Owen, McLean, Pamela, Issadore, David
Language: English
Subfield: Molecular Biology
Domain: Life Sciences
Open Access status: green
Funders: American Brain Foundation, American Academy of Neurology, Little Family Foundation, Turner Foundation, Directorate for Engineering, National Institute on Aging, National Institute of Allergy and Infectious Diseases, National Institute of Neurological Disorders and Stroke, Division of Materials Research
OpenAlex ID: W7140100769
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