Work

Protocol for acute oxidative stress induction in third-instar Drosophila melanogaster larvae

Year: 2026

Type: article

Abstract: Here, we present a protocol for inducing oxidative stress in the Drosophila melanogaster larval brain to facilitate the screening of regulators involved in redox homeostasis. We describe steps for the collection of synchronized larvae and their treatment with oxidative stressors. We then detail procedures for larval brain dissection, lipid droplet staining, image acquisition, and the assessment of glutathione redox status. For complete details on the use and execution of this protocol, please refer to Li et al. 1

Source: STAR Protocols

DOI: 10.1016/j.xpro.2026.104466

Authors: Jinhua Li, Yanzhe Pan, Yansong Zhang, Siyuan Lin, Min Liu, Alan Jian Zhu

Institutions: Peking University, State Key Laboratory of Membrane Biology, Center for Life Sciences

Language: English

Topic: Redox biology and oxidative stress

Subfield: Molecular Biology

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

SDG: Good health and well-being

Open Access status: gold

Estimated APC paid: $1,550