Year: 2026
Type: article
Abstract: Fluorescent unnatural amino acids (FlAAs) provide a powerful molecular bridge between small-molecule fluorophores and biomacromolecules, enabling minimally perturbative optical interrogation of peptides and proteins. Over the past decade, substantial progress has been made in the rational design, synthesis, and application of FlAAs, driven by advances in organic synthesis, C-H functionalization, and translation-based incorporation technologies. This review summarizes contemporary strategies for constructing FlAAs, with a particular emphasis on structure-property relationships and modular design principles. Beyond chemical synthesis, we summarize translation-machinery-mediated strategies, including chemical tRNA aminoacylation, flexizyme-based charging, and genetic code expansion, which collectively enable the incorporation of FlAAs into peptides and proteins in vitro and in living cells. Finally, we showcase representative applications of FlAAs in live-cell imaging, super-resolution microscopy, and photodynamic therapy, illustrating how compact fluorescent amino acids can serve as both imaging reporters and functional photoactive units. Together, this review summarizes current molecular design strategies for FlAAs and discusses how chemical modification of amino acid scaffolds can be linked to specific biological applications.
Source: Chemical & Biomedical Imaging
DOI: 10.1021/cbmi.6c00017
Institutions: Nanjing University, University of Edinburgh
Language: English
Subfield: Organic Chemistry
Field: Chemistry
Domain: Physical Sciences
Open Access status: diamond
OpenAlex ID: W7140183467