Work

Development of a novel duplex recombinase polymerase amplification (DRPA) assay for rapid detection of carbapenem-resistant Acinetobacter baumannii

Year: 2026

Type: article

Abstract: Carbapenem-Resistant Acinetobacter baumannii (CRAB) poses a major public health threat due to its multidrug resistance and high mortality rates, necessitating rapid and accurate diagnostic tools for timely intervention and infection control. This study developed and evaluated a novel detection method combining duplex recombinase polymerase amplification (DRPA) with a lateral flow strip (LFS) for the rapid visual identification of CRAB. The DRPA-LFS assay targets bla OXA−23 and bla OXA−51 genes, enabling isothermal amplification at 37 °C followed by a simple visual readout. The method demonstrated high sensitivity (10 2 CFU). Results can be obtained within 40 min without the need for complex instrumentation, making it suitable for resource-limited environments. the method offers a portable, user-friendly, and cost-effective solution for CRAB detection.

Source: BMC Infectious Diseases

DOI: 10.1186/s12879-026-13175-z

Authors: Chenyan Yuan, Ting Zhang, Juan Huo, Wenxue Liang, Lei Wang, Kun Wang

Institutions: Zhongda Hospital Southeast University, The First People’s Hospital of Lianyungang, Nantong University

Language: English

Topic: Biosensors and Analytical Detection

Subfield: Biomedical Engineering

Field: Engineering

Domain: Physical Sciences

SDG: Good health and well-being

Open Access status: gold

Estimated APC paid: $3,090