Enhancement of Endoglucanase Activity through Incorporation of Physical, Chemical, and Combined Mutagenesis in Thermophilic Strain of Aspergillus fumigatus
Year: 2026
Type: article
Abstract: An indigenous Aspergillus fumigatus strain was subjected to physical, chemical, and combined mutagenesis to enhance endoglucanase production. Using rice straw as a greater carbon source, maximum activity (84.94 U mL–1) was achieved via 10-minute UV exposure, while 150 µg mL–1 ethyl methane sulphonate (EMS) yielded 62.89 U mL–1. Combined mutagenesis (10 min UV+ 150 µg mL–1 EMS) significantly improved activity to 123.32 U mL–1, a 2.2-fold increase over the wild type. Optimization through response surface methodology (RSM) identified optimal conditions: pH 5.0, 55 °C, 5-day incubation, and 5 mL inoculum. Purified endoglucanase exhibited high affinity for CMC and was further activated by 300 mM CaCl2 to 176.92 U mL–1. Kinetic and characterization studies confirm that this thermophilic, genetically improved (physical, chemical, and combined mutagenesis) strain is highly suitable for industrial applications in textiles and biofuels.
Institution: University of Gujrat
Language: English
Subfield: Biotechnology
Domain: Life Sciences
Open Access status: diamond
OpenAlex ID: W7140227417
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