Pinhole Engineering based Enhanced Resolution (PEER) for Fluorescence Lifetime Imaging Microscopy
Year: 2026
Type: software
Abstract: This Python script streamlines the PEER workflow: it ingests six TIFF images—intensity (I) and phasor coordinates (G and S) captured with both the small‑pinhole and large‑pinhole settings of a confocal FLIM system—and automatically generates four figures: phasor‑density plots and intensity‑weighted lifetime maps for the confocal and PEER channels. The resulting PNG files — “phasor_confocal.png,” “phasor_peer.png,” “flim_confocal.png,” and “flim_peer.png” — are saved in the specified results folder.
Source: Code Ocean
Institution: Harvard University
Language: English
Subfield: Biophysics
Domain: Life Sciences
Open Access status: green
OpenAlex ID: W7140274175