Figure 2 from Ancestrally Diverse Autologous Patient-Derived Organoid–Immune Cell Coculture Platform for Addressing Immunotherapeutic Outcome Disparities in High-Grade Endometrial Cancer
Year: 2026
Type: other
Abstract: IFNγ pretreatment enhances cancer–immune cell interactions. A, A schematic depicting the experimental flow of organoid–immune cell coculture. EC, endometrial cancer. B, Raw colocalization coefficient between PDOs and caspase-3/7 signal from vehicle- and IFNγ-treated group (no immune cells) at the end point (86 hours). C, Representative images of coculture using a HGEC PDO with or without IFNγ prestimulation and autologous PBMC, CD8, and CD4 T cells. Red and green fluorescence represent endometrial organoids and corresponding immune cells, respectively. Blue fluorescence represents active caspase-3 signals. Scale bar, 70 μm. D, Colocalization of PDOs and immune cells over the time of imaging. Colocalization coefficient (R1) was calculated as described previously (26) and normalized to the initial time point. The area under the curve was used for statistical significance (ordinary t test). E, The end point viability of PDOs in the coculture measured by colocalization coefficient between organoid and caspase-3 signals. Colocalization coefficient was calculated as described previously (26) and normalized to no immune cell control (vehicle). Cas3, caspase-3. Ordinary one-way ANOVA (E) was used to calculate statistical significance (**, P < 0.01; ***, P < 0.001; ****, P < 0.0001).
Subfield: Oncology
Field: Medicine
Domain: Health Sciences
Open Access status: gold
OpenAlex ID: W7140276306
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