Figure 3 from Ancestrally Diverse Autologous Patient-Derived Organoid–Immune Cell Coculture Platform for Addressing Immunotherapeutic Outcome Disparities in High-Grade Endometrial Cancer
Year: 2026
Type: other
Abstract: Enhanced MHC expression mediated by EZH2 inhibition in HGEC organoids augments cancer–T cell interactions. A, Representative images of coculture using a HGEC PDO with or without TAZ prestimulation and autologous PBMC, CD8, and CD4 T cells. Scale bar, 70 μm. B, Colocalization of PDOs and immune cells over the time of imaging. Colocalization coefficient (R1) was calculated as described previously (26) and normalized to the initial time point. The area under the curve was used for statistical significance (ordinary t test). C, End point viability of PDOs in autologous coculture measured by colocalization coefficient between organoid and caspase-3 signals. Colocalization coefficient was calculated as described previously (26) and normalized to no immune cell control (vehicle). Cas3, caspase-3. Ordinary one-way ANOVA (C) was used to calculate statistical significance (****, P < 0.0001).
Subfield: Oncology
Field: Medicine
Domain: Health Sciences
Open Access status: gold
OpenAlex ID: W7140281118
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