Figure 4 from Ancestrally Diverse Autologous Patient-Derived Organoid–Immune Cell Coculture Platform for Addressing Immunotherapeutic Outcome Disparities in High-Grade Endometrial Cancer
Year: 2026
Type: other
Abstract: Organoid–NK cell coculture and autologous MMRp and MMRd organoid coculture validate our platform. A, Representative images of allogeneic NK cell coculture using a HGEC PDO line with or without IFNγ prestimulation. Red and green fluorescence represent HGEC PDOs and corresponding immune cells, respectively. Blue fluorescence represents active caspase 3 signals. Scale bar, 70 μm. B, Colocalization of PDOs and immune cells over the time of imaging. The area under the curve was used for statistical significance (ordinary t test). C, The end point viability of PDOs in the coculture measured by colocalization coefficient between organoid and caspase-3 signals. D, Representative images of coculture using MMRd and MMRp HGEC PDOs and autologous PBMCs. Scale bar, 70 μm. E, Quantification of MHC-I, -II, and PD-L1 protein expression levels of the MMRd and MMRp HGEC PDO lines measured by flow cytometry. MFI, mean fluorescent intensity. F, Colocalization of PDOs and immune cells at 14 hours of coculture period. G, Viability of the MMRd PDO in the coculture measured by colocalization coefficient between organoid and caspase-3 signals at 12 and 20 hours coculture period. H, Viability of the MMRp PDO in the coculture measured by colocalization coefficient between organoid and caspase-3 signals at 12 and 20 hours coculture period. Cas3, caspase-3. Ordinary one-way ANOVA (C, G, and H) and t test (F) were used to calculate statistical significance (*, P < 0.05; **, P < 0.01; ***, P < 0.001; ****, P < 0.0001).
Subfield: Oncology
Field: Medicine
Domain: Health Sciences
Open Access status: gold
OpenAlex ID: W7140327042
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