Work

Characterisation of the signal to noise ratio of 2-photon microscopes

Year: 2026

Type: article

Abstract: The signal to noise ratio (SNR) is a key performance metric of an imaging instrument. Here we describe the characterisation of SNR of a custom 2-photon microscope and compare the SNR performance of our microscope with selected commercial 2-photon microscopes. The methodology described in this paper can serve as guidance for others wishing to characterise and benchmark their 2-photon or other point-scanning microscopes.

Source: Scientific Reports

DOI: 10.1038/s41598-026-45224-7

Authors: Radek Macháň, Shau Poh Chong, Khee Leong Lee, Peter Török

Institutions: Nanyang Technological University, Singapore Centre for Environmental Life Sciences Engineering, Institute for Digital Molecular Analytics and Science

Language: English

Topic: Advanced Fluorescence Microscopy Techniques

Subfield: Biophysics

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

Open Access status: gold

Estimated APC paid: $2,690

Funders: Nanyang Technological University, Lee Kong Chian School of Medicine, Nanyang Technological University