Work

Protocol to identify SINE-VNTR-Alu regulators using genome-wide screening in human K562 cells

Year: 2026

Type: article

Abstract: The composite transposon SINE-VNTR-Alu (SVA) is hominid-specific and composed of five parts, but the regulatory mechanism of SVA transcription is still unclear. Here, we present a protocol to identify SVA regulators using genome-wide screening in human K562 cells. We describe steps for constructing an SVA-GFP reporter and performing genome-wide CRISPR-Cas9 screening in human K562 cells to identify genes that control SVA transcription. This protocol provides a representative procedure of genome-wide screening for transposon regulators. For complete details on the use and execution of this protocol, please refer to Zhou et al. 1

Source: STAR Protocols

DOI: 10.1016/j.xpro.2026.104468

Authors: Ziqiang Zhou, Shicong Zhu, Deng Pan, Hsiang-Ying Lee, Nian Liu

Institutions: Center for Life Sciences, Tsinghua University, Peking University, State Key Laboratory of Membrane Biology

Language: English

Topic: Chromosomal and Genetic Variations

Subfield: Plant Science

Field: Agricultural and Biological Sciences

Domain: Life Sciences

SDG: Good health and well-being

Open Access status: gold

Estimated APC paid: $1,550