Work

Viscoelastic and Morphologic Changes in the Lumbar Intervertebral Discs of the 5xFAD Mouse Model of Alzheimer's Disease

Year: 2026

Type: article

Abstract: ABSTRACT Background Chronic low back pain, commonly associated with intervertebral disc (IVD) degeneration, is highly prevalent in patients with Alzheimer's disease (AD), and the severity of the pain is correlated with the severity of the dementia. While incidences of both afflictions increase dramatically in the elderly population, it is unknown whether AD exacerbates the health of the IVD. Aims The aim of this study is to examine how AD‐related mutations affect the structure and mechanics of the IVD. Materials and Methods Utilizing one‐year‐old male and female 5xFAD mice that constitutively express human APP and PSEN1 transgenes with five AD‐linked mutations, we measured the lumbar IVD's extracellular matrix composition, the three‐dimensional structure, histopathological degeneration, and mechanical behaviour. Results The amount of collagen, glycosaminoglycans, and advanced glycation end‐products in the IVD did not differ in the 5xFAD animals. Likewise, the 5xFAD IVDs were not histopathologically degenerated. However, the IVD volume, measured by contrast‐enhanced microCT, was larger in the 5xFAD animals ( p = 0.046; 1–β = 0.53; Cohen's d = 1.19). Morphologically, the 5xFAD IVD heights were homogeneous and flatter than the WT IVDs ( p = 0.043; 1–β = 0.60; Cohen's d = 1.41). Finally, dynamic micro compression revealed that 5xFAD IVDs have higher loss tangent ( p = 0.0062; 1–β = 0.85; Cohen's d = 1.60) and greater energy dissipation ( p = 0.0379; 1–β = 0.55; Cohen's d = 1.21). Discussion Shifts in viscoelastic behaviour and disc morphology are associated with early tissue degradation and affect the IVD's ability to efficiently recover from mechanical loading, predisposing the IVD to eventual degeneration. Conclusion This work suggests that IVD's structure, morphology, and mechanics are affected by AD. Future work will focus on defining the molecular mechanisms of these changes in the IVD and their consequences in individuals with AD.

Source: JOR Spine

DOI: 10.1002/jsp2.70171

Authors: C. E. Gonzalez, C. A. Schurman, K. A. Wilson, B. Schilling, L. M. Ellerby, S. Y. Tang

Institutions: Washington University in St. Louis, Buck Institute for Research on Aging

Language: English

Topic: Spine and Intervertebral Disc Pathology

Subfield: Pathology and Forensic Medicine

Field: Medicine

Domain: Health Sciences

SDG: Good health and well-being

Open Access status: gold

Estimated APC paid: $2,480