Work

Deletion of Cab39 adaptors results in KS-WNK1 independent accumulation of SPAK in biomolecular condensates

Year: 2026

Type: article

Abstract: In this study, we identify novel biomolecular condensates (puncta) that appear in the absence of KS-WNK1, a component of WNK bodies. Mice knockout for both KS-WNK1 and Cab39 adaptor proteins exhibit large SPAK-containing puncta that also comprise WNK4 and L-WNK1. These puncta are p62-positive and ubiquitin-negative, indicating that they are sequestrating rather than degrading structures. Formation of these puncta requires active phosphorylation, as they are not observed in mice fed with a high K + diet.

Source: American Journal of Physiology-Renal Physiology

DOI: 10.1152/ajprenal.00491.2025

Authors: Mohammed Zubaerul Ferdaus, Masa-Ki Inoue, Andrew S. Terker, Paul A. Welling, Eric Delpire

Institutions: Vanderbilt University, Johns Hopkins University

Language: English

Topic: Ion Transport and Channel Regulation

Subfield: Molecular Biology

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

Open Access status: hybrid

Funders: Fondation Leducq, National Institutes of Health, National Institute of Diabetes and Digestive and Kidney Diseases