Work

Culturing a discerning palette for spatiofunctional profiling

Year: 2026

Type: review

Abstract: Recent innovations in proteomics have opened a window into how proteins distribute within cells. However, spatial reconnaissance is often not sufficient to inform on mechanism. Such information also does not inherently suggest how to functionally perturb proteins in specific regions, which is ultimately required for biological studies. We propose a new concept, spatiofunctional profiling that uses (preter)natural metabolites to census proteins that have the proclivity to engage with specific small-molecule ligands in a specific locale. Based on our studies on signaling by reactive (preter)natural metabolites, this strategy will index proteins within a specific locale that are susceptible to chemical manipulation. • Quantitative mapping of chemical actionability of local proteomes. • Challenges and limitations. • Translational applications and future prospects.

Source: Current Opinion in Chemical Biology

DOI: 10.1016/j.cbpa.2026.102669

Authors: Marcus J.C. Long, Xin Liang, Yimon Aye

Institutions: University of Oxford, British Heart Foundation

Language: English

Topic: Advanced Proteomics Techniques and Applications

Subfield: Spectroscopy

Field: Chemistry

Domain: Physical Sciences

Open Access status: hybrid

Estimated APC paid: $4,510

Funders: Schweizerischer Nationalfonds zur Förderung der Wissenschaftlichen Forschung, European Research Council