Single‐Injection Multi‐Omics Analysis by Direct Infusion Mass Spectrometry
Year: 2026
Type: article
Abstract: Combined proteomics, metabolomics, and lipidomics analyses require long liquid chromatography-mass spectrometry (LC-MS) run times, limiting throughput and increasing costs for large-scale studies. Here, we present single-injection multi-omics analysis by direct infusion (SMAD), an integrated platform leveraging ion mobility mass spectrometry and self-developed software tools to enable single injection multi-omics analysis without liquid chromatography. SMAD allows quantification of over 9000 metabolite m/z features and over 1300 proteins from the same sample in less than 5 min. We validated the efficiency and reliability of SMAD with three case studies: (1) mouse macrophages after M1/M2 polarization and senescence, (2) a pilot drug screen in human cells, and (3) large-scale high-throughput drug screening of mammalian cells in 96-well plates. Finally, relationships between proteomic and metabolomic data are discovered by machine learning and validated.
Language: English
Subfield: Molecular Biology
Domain: Life Sciences
Open Access status: hybrid
Estimated APC paid: $5,510
Awards: G3473901384, G5529494987, G6184508185, Drug Discovery for Alzheimer’s Disease Enabled by Multi-Omics and Artificial Intelligence, G7477658958, G9000237079, Democratizing Multi-Omics to Expedite Discovery of Hidden Metabolic Pathways, Exploring the fundamental cellular mechanisms driving cellular senescence in macrophages
OpenAlex ID: W7143314814