Work

Preventing peri-implantitis with Ag-Est816 nanocomposite: modified rat model and preventive assessment

Year: 2026

Type: article

Abstract: Background Peri-implantitis is a prevalent complication leading to progressive bone loss around dental implants, with current therapeutic strategies offering limited preventive efficacy. Objective This study aimed to develop a modified rat model of peri-implantitis to evaluate the effects of a silver nanoparticle and quorum quenching enzyme nanocomposite (Ag-Est816) on peri-implant bone health. Methods The research comprised two parts: (A) development of the peri-implantitis rat model, and (B) assessment of Ag-Est816's preventive effect. In Part A, titanium implants infected with Porphyromonas gingivalis and Streptococcus sanguinis were surgically placed into the femurs of five male Sprague-Dawley rats weighing 180–220 g. After four weeks, peri-implant bone loss was evaluated using micro-computed tomography (Micro-CT) to measure bone volume to total volume ratio (BV/TV), and histological analysis was performed to assess bone architecture. Non-infected implants served as controls. In Part B, Ag-Est816 was applied to infected titanium implants, while control implants were treated with sterilized phosphate-buffered saline (PBS). Micro-CT and histological analysis were used to evaluate bone loss and tissue integrity. Results In Part A, the BV/TV of infected implants and controls was 6.9 ± 1.4% vs. 32.8 ± 9.0% ( p < 0.001). Histological analysis revealed disorganized collagen, impaired mineralization, and stalled bone formation at the implant interface in the infected samples, in contrast to the ordered, mature osseointegration observed in the controls. In Part B, the BV/TV of Ag-Est816-treated implants and controls was 23.6 ± 3.3% vs. 5.1 ± 1.8% ( p < 0.001). Histological analysis revealed new bone regeneration with trabecular integrity around Ag-Est816-treated implants, in contrast to compromised osseointegration with disrupted, sparse bone and fibrous, inflammatory tissue in the controls. Conclusions A modified rat femur model of peri-implantitis was successfully developed. Ag-Est816 effectively prevented peri-implant bone loss.

Source: Frontiers in Oral Health

DOI: 10.3389/froh.2026.1781671

Authors: Zelda Ziyi Zhao, Mengyin Luan, Chun Hung Chu, Jing Zhang

Institutions: University of Hong Kong, Anhui Medical University

Language: English

Topic: Dental Implant Techniques and Outcomes

Subfield: Oral Surgery

Field: Dentistry

Domain: Health Sciences

SDG: Good health and well-being

Open Access status: gold

Estimated APC paid: $3,077