Hyperosmotic stress induces PARP1 ‐mediated HPF1 ‐dependent mono( ADP ‐ribosyl)ation
Year: 2026
Type: article
Abstract: While the downstream effectors of the hyperosmotic stress response are relatively well characterized, the primary molecular sensors responsible for initial stress detection remain poorly defined. In this study, we demonstrate that hyperosmotic stress triggers a rapid and transient mono(ADP-ribosyl)ation (MARylation). Beside MARylation, signs of acute genotoxicity are missing and CHK1 activation is observed only upon recovery from osmotic stress. Our data indicate that PARP1 catalyzes its own MARylation in an HPF1 co-factor dependent manner. Biochemical assays further demonstrate that the mono-ADP-ribose moiety is resistant to hydroxylamine treatment, which is a feature of HPF1-directed O-glycosidic bonds. Together, these findings support a model in which PARP1 acts as a sensor of chromatin structure changes induced by hyperosmotic stress leading to its autoMARylation.
Source: FEBS Letters
Institutions: University of Szeged, HUN-REN Szegedi Biológiai Kutatóközpont, Institute of Genetics, Centre National de la Recherche Scientifique, Institut de génétique et de développement de Rennes, Structure Fédérative de Recherche en Biologie et Santé de Rennes, Université de Rennes, University of Oxford
Language: English
Subfield: Oncology
Field: Medicine
Domain: Health Sciences
Open Access status: hybrid
Estimated APC paid: $3,980
Awards: G1718726484, G1888827638, G230613184, Reversible ADP-ribosylation signaling in the control of coronavirus infection, G309025395, ADP-ribosylation signalling in genome stability, Assessing the functions of histone ADP-ribosylation during DNA repair, ADP-ribosylation of DNA in Mycobacterium tuberculosis, Understanding the activity and role of DarTG, a toxin:antitoxin system responsible for a novel DNA modification
OpenAlex ID: W7144225704