Work

Circular Dichroism Reveals Positive Cooperativity through Compensatory Motion in Stepwise Cage Metalation

Year: 2026

Type: article

Abstract: High Resolution Image Download MS PowerPoint Slide Biological allosteric systems achieve functional control through long-range communication between distant binding sites. Here, we report that a symmetric chiral cage bearing two equivalent tris(2-pyridylmethyl)amine ( TPMA ) binding sites exhibits structural communication reminiscent of allosteric behavior during stepwise Zn(II) coordination. NMR titrations reveal sequential metalation kinetics in which the second coordination step becomes comparatively slower in mixed solvents, consistent with electrostatic effects and structural preorganization. Circular dichroism (CD) spectroscopy tracks the progressive structural evolution of the cage, with five isodichroic points confirming clean interconversion between discrete species. TD-DFT calculations reproduce the experimental chiroptical evolution, and correlate the observed spectral changes with systematic geometric reorganization of the framework over distances exceeding 10 Å. This work illustrates the potential of CD spectroscopy as a diagnostic tool for probing allosteric-like mechanisms in synthetic supramolecular systems, with implications for heterometallic catalysis, responsive materials, and allosteric synthetic receptors.

Source: Inorganic Chemistry

DOI: 10.1021/acs.inorgchem.6c00988

Authors: Melvin Raulin, Chiara Slaviero, Federico Begato, Giulia Licini, Cristiano Zonta