Differentiation of Adipose Derived Stem Cells by Stimulating with Extracellular Vesicles Secreted from Dermal Papilla Cells Activated with Osteogenic and Adipogenic Inducers
Year: 2022
Type: dissertation
Abstract: Dermal papilla ceils (DPCs) exist as spheroidal or globular shape m In-vivo condition and play crucial roles in hair regeneration, but they readily lose their hair forming ability during in vitro culture.Although the formation of spheroids partially restores the ability, shrinkage of the spheroids makes it difficult to maintain cellular viability.To address this problem, we stimulated DPCs with factors known to induce adipogenic and/or osteogenic differentiation, because DPCs share unique gene expression profiles with adipocytes and osteocytes.We isolated DPCs from versican (vcan)-GFP mice, in which GFP is expressed under the control of a vcan promoter, which is strongly active in DPCs of anagen hair follicles.In shortly, versican is a proteoglycan sulfate and serve the purpose as ECM (extracellular matrix molecule to cell-cell attachment.Isolated intact DPCs from anagen hair follicle showed intense GFP-fluorescence intensity, but it lost the fluorescence while these spheroids attached in monolayer culture surface.Even though in spheroid culture system, fluorescence getting declined and spheroid shaped became shrink which indicated the lost of hair inductive properties in DPCs.However, we analyze GFP fluorescence was most intense when the spheroids were made from DPCs cultured in a half-diluted combination of adipogenic and osteogenic media (CAO1/2), a Dulbecco's modified Eagle's medium-based medium (DMEM) that contains 10% FBS, 275 nM dexamethasone, 2.5 mM -glycerol phosphate, 12.5 g/mL ascorbic acid, 0.125 M Isobutylmethylxanthine and 2.5 ng/mL insulin.The dose of each additive used was less than the optimal dose for adipogenic or osteogenic differentiation.We observed that, the media named CAOl/2 able to maintain GFP-fluorescence compare with the control DPCs which cultured with only 10% FBS contain DMEM, but the DPCs spheroid
Author: Taheruzzaman Kazi
Language: English
Topic: Hair Growth and Disorders
Subfield: Dermatology
Field: Medicine
Domain: Health Sciences
Open Access status: green
Funder: Shimane University
OpenAlex ID: W7146628797