Pyrazole-Thiazole Hybrids: Synthesis and Biological Evaluation against Trypanosoma cruzi and Mycobacterium tuberculosis

Year: 2026

Type: article

Abstract: High Resolution Image Download MS PowerPoint Slide Pyrazole–thiazole hybrids 1(a–k) were synthesized and fully characterized to investigate their biological activities against Trypanosoma cruzi and Mycobacterium tuberculosis . Physicochemical profiling confirmed favorable drug-like properties across the series. Biological assays identified compound 1g (3-F) as the most active against T. cruzi, with IC 50 values of 11.66 μM (SI > 42.8) in trypomastigotes and 26.83 μM (SI > 18.9) in amastigotes. Compound 1h (4-Br) displayed significant antimycobacterial activity, with a MIC of 32.28 μM against the H37Rv strain. In silico analyses suggested preferential stabilization of halogenated derivatives through hydrophobic and π-driven interactions in structurally related enzymatic pockets, used here as an exploratory model. Taken together, these findings demonstrate that halogen substitution strongly modulates affinity and biological performance, supporting pyrazole–thiazole hybrids as promising scaffolds for further optimization against neglected-parasite pathogens. Future studies expanding halogen patterns and probing alternative binding pockets may improve potency and target selectivity across this chemical space.

Source: ACS Omega

DOI: 10.1021/acsomega.5c12469

Authors: Cynthia Nathália Pereira, Lorraine Martins Rocha Orlando, Edinaldo Castro de Oliveira, Mirian Claudia de Souza Pereira, Christian S. Canales Carnero, Oswaldo Julio Ramirez Delgado, Cesar Augusto Roque-Borda, Fernando Rogério Pavan, Maurício Silva dos Santos

Institutions: Universidade Federal de Itajubá, Fundação Oswaldo Cruz, National University of Engineering, Zimmer Biomet (Germany), National University of Engineering, Universidade Estadual Paulista (Unesp), Catholic University of Santa María

Language: English

Topic: Synthesis and biological activity

Subfield: Organic Chemistry

Field: Chemistry

Domain: Physical Sciences

SDG: Good health and well-being

Open Access status: gold

Estimated APC paid: $1,935

Funders: Fundação de Amparo à Pesquisa do Estado de São Paulo, Coordenação de Aperfeiçoamento de Pessoal de Nível Superior, Conselho Nacional de Desenvolvimento Científico e Tecnológico, Fundação Carlos Chagas Filho de Amparo à Pesquisa do Estado do Rio de Janeiro, Fundação de Amparo à Pesquisa do Estado de Minas Gerais, Fundação Oswaldo Cruz, Consejo Nacional de Ciencia, Tecnología e Innovación Tecnológica

OpenAlex ID: W7146989701