Supplemental Figure 8 from Defective DNA Damage Response Is a Targetable Therapeutic Vulnerability in ESR1-Mutant Breast Cancer
Year: 2026
Type: supplementary-materials
Abstract: BrdU uptake is attenuated in Y537C cells using Chk1i as a single agent or in combination with Olap under replication stress induced by HU Percent BrdU uptake in Y537C cells analyzed by flow cytometry. Bars represent the average + standard deviation of percentage of BrdU+ cells relative to total propidium iodide positive cells (n=2). Significance was calculated using two-way ANOVA corrected for multiple comparisons to compare treated cells to Ctrl conditions, *p<0.05, ****p<0.0001. Cells were treated for 24 hours of Chk1i (500 nM) or Chk1i+Olap (5 µM) followed by 24 hours of 0.5 mM HU in combination with indicated drug treatments as applicable. All treatments were performed in charcoal stripped media after 48 hours of estrogen deprivation in charcoal stripped media followed by 36 hours of Lovastatin (10 µM) to synchronize cell cycles before inhibitor treatment.
Authors: Sarah K. Herzog, Jessica H. Stevens, Guowei Gu, Sandra L. Grimm, Kloma Cardoza, Autumn G.M. Hawkins, Hangqing Lin, Daniela Ramos, Amanda R. Beyer, David G. Edwards, Derek Dustin, Harry J. Yang, Nicole Liang, Ashfia F. Khan, Tasneem Bawa-Khalfe, Daniel J. McGrail, Shiaw-Yih Lin, Cristian Coarfa, Suzanne A.W. Fuqua
Language: English
Subfield: Pulmonary and Respiratory Medicine
Field: Medicine
Domain: Health Sciences
Open Access status: gold
OpenAlex ID: W7147259477