Supplemental Figure 19 from Defective DNA Damage Response Is a Targetable Therapeutic Vulnerability in ESR1-Mutant Breast Cancer
Year: 2026
Type: supplementary-materials
Abstract: Olap increases chromatin retention of PARP a. Western blot analysis showing the chromatin bound fraction of MCF-7 WT, Y537S, Y537C and F+A cells after 48 hours of estrogen deprivation in charcoal stripped serum media followed by 48 hours of combination treatment with 10 nM Fulvestrant and 5 uM Olaparib compared to untreated controls. b. Western blot analysis showing the chromatin bound fraction of MCF-7 WT, Y537S, Y537C and F+A cells after 48 hours of estrogen deprivation in charcoal stripped serum media followed by 72 hours of treatment with 5 uM Olaparib or 5 uM Veliparib, isolated with the ThermoFisher subcellular protein fractionation kit.
Authors: Sarah K. Herzog, Jessica H. Stevens, Guowei Gu, Sandra L. Grimm, Kloma Cardoza, Autumn G.M. Hawkins, Hangqing Lin, Daniela Ramos, Amanda R. Beyer, David G. Edwards, Derek Dustin, Harry J. Yang, Nicole Liang, Ashfia F. Khan, Tasneem Bawa-Khalfe, Daniel J. McGrail, Shiaw-Yih Lin, Cristian Coarfa, Suzanne A.W. Fuqua
Language: English
Subfield: Oncology
Field: Medicine
Domain: Health Sciences
Open Access status: gold
OpenAlex ID: W7147573743