Work

Data from: HIV-1 Vif disrupts phosphatase feedback regulation at the kinetochore, leading to a pronounced pseudo-metaphase arrest

Year: 2025

Type: dataset

Abstract: This dataset supports the following findings: the HIV-1 Virion Infectivity Factor (Vif) promotes viral infection by targeting and degrading cellular APOBEC3 proteins, which are key regulators of intrinsic and innate antiviral immunity. Although Vif is also known to induce cell cycle arrest, its precise effects on the cell cycle have remained unclear. Using high-temporal-resolution single-cell live imaging and super-resolution microscopy, we found that Vif does not disrupt the G2/M boundary as previously proposed. Instead, Vif induces a distinct and robust pseudo-metaphase arrest, unlike the mild prometaphase arrest caused by Vpr. During this arrest, chromosomes initially align at the metaphase plate but later lose alignment, producing polar chromosomes. Notably, Vif markedly reduces Protein Phosphatase 1 (PP1) and Protein Phosphatase 2A (PP2A) levels at kinetochores, unlike Vpr. These findings reveal a novel role for Vif in kinetochore regulation and chromosome organization during mitosis.

Source: DRYAD

DOI: 10.5061/dryad.k6djh9wj7

Authors: Suzuki, Aussie, Sherer, Nathan

Institution: University of Wisconsin–Madison

Language: English

Topic: Microtubule and mitosis dynamics

Subfield: Cell Biology

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

SDG: Good health and well-being

Open Access status: green

Funders: National Institute of General Medical Sciences, National Institute of Allergy and Infectious Diseases