The pangenome: a statistical model, not a fixed biological property
Year: 2026
Type: article
Abstract: The pangenome, representing the complete genetic repertoire of a species, has become a central concept in modern prokaryotic genomics, moving the field beyond the limitations of a single reference genome. This review provides a comprehensive overview of the pangenomic landscape, from its conceptual foundations to its diverse applications. We critically examine the methodological and statistical underpinnings of pangenome analysis, emphasizing that the pangenome is not a fixed biological entity but a statistical model whose outputs are fundamentally dependent on dataset quality, curation, and taxonomic resolution. We discuss the transition from the simple open/closed dichotomy to more nuanced, ecologically driven models like pangenome fluidity and the ecogenome, which better explain the evolutionary dynamics of prokaryotic genomes. With a special focus on the archaeal pangenome, we highlight the unique challenges and novel evolutionary insights emerging from this under-explored domain. As the field moves towards graph-based representations, AI-driven analysis, and the expansion into metapangenomics integrated with functional multi-omics, we argue that a rigorous and critical approach to data interpretation will be paramount to unlocking a true understanding of microbial evolution and function.
Source: Bioinformatics Advances
Author: Abraham Guerra
Language: English
Subfield: Molecular Biology
Domain: Life Sciences
Open Access status: gold
Estimated APC paid: $2,726
OpenAlex ID: W7148312239