Effect and Molecular Mechanism of Medium Chain Triglyceride on Regulating Rat Lipid Metabolism(中链甘油三酯调控大鼠脂代谢的作用及其分子机制)
Year: 2025
Type: article
Abstract: Medium chain triglyceride (MCT) is dietary fat naturally existed in palm oil and breast milk. As a novel functional ingredient, it can be used in the production of formula food for special medical purpose. However, the specific target for MCT regulating lipid metabolism is still unclear. Based on network pharmacology analysis and rat model validation, the potential role and target of MCT for regulating lipid metabolism were explored. Animal model demonstrated that no hepatorenal toxicity in rat was observed after nutritional intervention of MCT, indicating its good edible safety. And MCT nutritional intervention down-regulated the levels of serum triglyceride, total cholesterol and low-density lipoprotein, and inhibited the accumulation of adipose tissue of the epididymis in rat. Network pharmacology analysis showed that there were 27 overlapping targets between MCT metabolites (octanoic acid and decanoic acid) and lipid metabolism-related genes, and potentially involved in regulation of peroxisome proliferator activated receptor lipid metabolism-related pathways and fatty acid binding and transport. Western blot assay also indicated that MCT could down-regulate the expression of fatty acid-binding protein 4 (FABP4) of the small intestine in a dose-dependent manner. Results indicated that the MCT nutritional intervention could down-regulate the expression of FABP4, subsequently affecting the absorption and transport of fatty acid, reducing the level of serum lipids and alleviating fat accumulation, thereby regulating the lipid metabolism. The purpose of this study was to clarify the role of MCT in regulating lipid metabolism and its potential targets, and to provide a theoretical basis for the application of MCT in lipid metabolism regulation.(中链甘油三酯(medium chain triglyceride,MCT)是天然存在于棕榈油、母乳中的膳食脂肪,作为新型功能配料可用于特殊医学用途配方食品等生产,但MCT发挥脂代谢调控作用的靶点尚不清晰。借助大鼠模型验证和网络药理学分析,探究了MCT调控脂代谢的潜在作用和靶点。动物模型研究证实:MCT营养干预大鼠未出现肝肾毒性,表明MCT具有良好的食用安全性；MCT营养干预降低了大鼠血清甘油三酯、总胆固醇和低密度脂蛋白水平,抑制了睾周脂肪堆积等。网络药理学分析显示,MCT代谢产物辛酸、癸酸的潜在作用靶点与脂代谢相关基因存在27个交集靶点,且潜在参与过氧化物酶体增殖物激活受体脂代谢相关通路及脂肪酸结合与转运调控。免疫印迹分析结果也表明,MCT可剂量依赖地下调小肠中脂肪酸结合蛋白4(FABP4)表达。研究结果表明,MCT营养干预可下调FABP4等蛋白表达继而影响脂肪酸吸收转运,并降低血脂水平、缓解脂肪堆积等,进而调节机体脂代谢。研究旨在明确MCT调控脂代谢的作用及其潜在靶点,为MCT应用于脂代谢调控提供理论依据。)
Institution: Beijing Technology and Business University
Language: Chinese
Subfield: Nutrition and Dietetics
Field: Nursing
Domain: Health Sciences
Open Access status: green
OpenAlex ID: W7148756191
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