Work

Enhancing tomato fruit sweetness by CRISPR/Cas9-mediated SlVIF gene editing

Year: 2026

Type: article

Abstract: A B S T R A C T In tomato plants, vacuolar invertase inhibitor (VIF) is a negative regulator of sucrose (SUC) degradation into fructose (FRU) and glucose (GLU), suggesting knockout of SlVIF promotes the accumulation of FRU and GLU. A dual-target vector of pKSE402- SlVIF was constructed and introduced into the tomato breeding parent ‘1912’ via Agrobacterium -mediated transformation. The editing efficiency was as high as 47%. Two Cas9-free T 1 lines exhibited delayed fruit ripening, significantly increased FRU and GLU levels, and reduced SUC content at the red ripe stage compared to wild-type (WT) plants, while fruit size and yield were unchanged. Additionally, in the SlVIF knockout mutants, the VIF protein structure was significantly altered, and VIN enzyme activity was markedly increased, which may underlie the enhanced degradation of sucrose in tomato fruits. The findings indicate that the sweetness of tomato fruit can be improved by CRISPR/Cas9-mediated SlVIF gene editing, which provides valuable resources for high-quality breeding in tomato.

Source: Plant Physiology and Biochemistry

DOI: 10.1016/j.plaphy.2026.111270