Work

Genomic characterization of rabies virus glycoprotein co-expressing CD70 CAR-T cells during killing of glioma cells in vitro

Year: 2026

Type: article

Abstract: Background Chimeric antigen receptor T cell therapy has limited efficacy in the treatment of glioma due to the blood-brain barrier and T cell exhaustion. Enhancement with rabies virus glycoprotein (RVG29) has shown improved brain-related efficacy. Methods In this study, we developed CD70R CAR-T cells by modifying anti-CD70 CAR-T cells with RVG29 and tested their tumor-killing ability in vitro . Results Transcriptomic profiling via RNA-seq revealed the activated signaling pathways in modified CD70R CAR-T cells. These cells displayed effective in vitro cytotoxicity against CD70 + ; glioma cells, furthermore, co-culture with glioma cells promoted the expansion of quiescent CD70R CAR-T cells. Conclusion The RVG29 modification enhances CAR-T therapy for brain tumors and holds promise for treating glioma.

Source: Frontiers in Immunology

DOI: 10.3389/fimmu.2026.1680513

Authors: Feng Ji, Kexing Gao, Xianchen Wu, Hao Lin

Institutions: Southeast University, Zhongda Hospital Southeast University, Southeast University

Language: English

Topic: CAR-T cell therapy research

Subfield: Oncology

Field: Medicine

Domain: Health Sciences

SDG: Good health and well-being

Open Access status: gold

Estimated APC paid: $3,801