Work

Increased PET 11C-PBR28 binding in multiple sclerosis normal-appearing white matter correlates with MRI measures of myelin loss

Year: 2026

Type: article

Abstract: Introduction The Positron Emission Tomography (PET) tracer 11 C-PBR28 binds to the Translocator Protein (TSPO), a marker of activated microglia. Advanced Magnetic Resonance (MR) techniques are sensitive to different tissue properties such as myelin (myelin water fraction, MWF; magnetisation transfer ratio, MTR; radial diffusivity, RD; choline, tCho), axons (axial diffusivity, AD; n-acetylaspartate, tNAA) and gliosis (myo-inositol, mI). Objective To compare 11 C-PBR28 binding across MS subtypes and investigate the correlation between 11 C-PBR28 binding and advanced MR measures in normal-appearing white matter (NAWM) and lesions. Methods Twelve people living with MS (7 relapsing-remitting MS (RRMS) and 5 progressive MS (PMS)) and 6 healthy controls (HC) were scanned with PET and MRI. A 70-minute 11 C-PBR28 PET session was used to determine the non-displaceable binding potential (BP ND ). Advanced 3T MR included myelin water imaging, diffusion tensor imaging and spectroscopy. Mean measurements were extracted from lesion and NAWM masks. Results Within NAWM, 11 C-PBR28 binding was different between groups (BP ND HC=0.70, RRMS=0.72, PMS=0.79; p=0.03) and lesions exhibited lower BP ND (NAWM=0.75, lesion=0.63; p=0.0002). Lesions also had lower MWF (NAWM=0.13, lesion=0.08) and MTR (NAWM=41.5, lesion=33.5), and higher AD (NAWM=1.05, lesion=1.41) and RD (NAWM=0.50, lesion=0.83) than NAWM (all p<0.0001). In NAWM, correlations were found between PBR-BP ND and MWF (R=-0.71, p=0.001) and MTR (R=-0.68, p=0.003). Conclusions Increased inflammation and gliosis indicated by elevated 11 C-PBR28 binding in NAWM are associated with demyelination (decreased MWF and MTR), especially in PMS. The ability to measure and monitor diffuse inflammation could help evaluate therapies designed to target whole brain immune activity and more specifically microglia.

Source: Multiple Sclerosis and Related Disorders

DOI: 10.1016/j.msard.2026.107189