Work

In vitro evaluation of the effects of flavonoids and saponins from Chenopodium bonus-henricus L. on non-enzyme-induced lipid peroxidation in isolated rat liver microsomes

Year: 2026

Type: article

Abstract: The aim of this study was to evaluate the effects of 15 saponins and flavonoids from the roots and aerial parts of C. bonus-henricus L. on non-enzymatic (Fe 2+ /AA)-induced lipid peroxidation in isolated rat liver microsomes. The production of MDA was used as a marker of lipid peroxidation. All saponins exhibited pronounced antioxidant activity (reducing MDA production by 17–23%) comparable to that of silymarin (20%), a well-established antioxidant and hepatoprotective agent. The bayogenin ( ChBh-04 ) and 2β-hydroxygypsogenin saponins showed the strongest effects. Flavonoids demonstrated significant antioxidant activity in microsomes from old rats, whereas their effects were less pronounced in microsomes from young rats. The highest reduction in MDA levels, exceeding that of silibinin (61%), was achieved by 6-methoxykaempferol glycoside (62%, ChBhnf-09 ), likely due to the ferulic acid in its moiety. These findings suggest that saponins and flavonoids from wild spinach may play a significant role in protecting liver cells from oxidative stress.

Source: Pharmacia

DOI: 10.3897/pharmacia.73.e188444

Authors: Magdalena Kondeva-Burdina, Paraskev T. Nedialkov, Zlatina Kokanova-Nedialkova

Institution: Medical University of Sofia

Language: English

Topic: Antioxidant Activity and Oxidative Stress

Subfield: Biochemistry

Field: Medicine

Domain: Health Sciences

SDG: Good health and well-being

Open Access status: gold

Estimated APC paid: $107