Label-free proteomics and single-cell mass cytometry analysis of 5 immune tissues from mice with artesunate administration reveal immunosuppression of artesunate via BST2 and MDSCs
Year: 2026
Type: article
Abstract: Artesunate is a worldwide prevalent antimalarial medicine, which has also been demonstrated for anti-tumor, anti-virus and anti-autoimmune diseases. Yet the underlying molecular and cellular mechanisms remain largely unexplored. Here, we examined immunological features of 5 immune tissues (spleen, bone marrow, thymus, lymph nodes and peritoneal cavity) of mice with artesunate administration through label-free proteomics and high-dimensional single-cell mass cytometry (CyTOF). Label-free proteomics uncovered dramatic alterations for the interferon-I (IFN-I) induced proteins, especially BST2 and IFIT1 downregulation in spleen and bone marrow. CyTOF analysis further indicated that the percentage of MDSCs was significantly elevated in spleen and bone marrow. The follow-up in vivo LPS-induced inflammatory model confirmed that pretreatment with artesunate attenuated the inflammatory damage, and the expression of BST2 was decreased in the spleen of mice with artesunate administration. Together, our findings presented an immune landscape of multiple tissues of mice with artesunate administration. These datasets provide comprehensive biological resources to develop therapeutic strategies for inflammatory diseases.
Source: Fundamental Research
Language: English
Subfield: Immunology
Field: Immunology and Microbiology
Domain: Life Sciences
Open Access status: diamond
Funders: Shanghai Municipal Health Commission, National Natural Science Foundation of China, Ministry of Science and Technology of the People's Republic of China, Shanghai Municipal Education Commission, Science and Technology Commission of Shanghai Municipality, Shanghai Jiao Tong University, Shanghai City Youth Science and Technology Star Project, National Key Research and Development Program of China
OpenAlex ID: W7153767650