Year: 2026
Type: article
Abstract: Enzyme-photo-coupled systems (EPCSs) offer a green strategy for producing high-value chemicals, but significant gaps remain in comparison to natural photosynthetic systems. Herein, we report an all-in-one EPCS (AiO-EPCS) by integrating photocatalytic (photocatalyst, P; electron mediator, M; electron donor, D) and biocatalytic (enzyme and cofactor) units in a hydrogen-bonded organic framework (HOF) for efficient NAD(P)H regeneration and light-driven enzymatic transformations, functioning like a chloroplast. The photocatalytic system assembled by incorporating P and M onto poly(allylamine hydrochloride) (PAH), PAHPM, exhibits an 11-fold enhanced photocatalytic NAD(P)H regeneration yield over the free mixture. Subsequently, two different AiO-EPCSs are fabricated by trapping two biocatalytic systems into HOF with the assistance of PAHPM. A double-layer architecture forms within the framework, partially separating the photo- and bio-catalytic modules while keeping all catalytic components in proximity. Moreover, adsorption of D onto HOF enables the system to be recycled by a single D addition, aligning AiO-EPCSs with natural photosynthesis in economic sustainability. Enzyme-photo-coupled systems (EPCSs) offer a green strategy for producing high-value chemicals. In this study, the authors report an all-in-one EPCS (AiO-EPCS) by integrating photocatalytic and biocatalytic (enzyme and cofactor) units into a hydrogen bonded organic framework, for NAD(P)H regeneration and light-driven enzymatic transformations.
Source: Nature Communications
Institutions: Tianjin University, UNSW Sydney
Language: English
Subfield: Molecular Biology
Domain: Life Sciences
Open Access status: gold
Estimated APC paid: $6,990
Award: G4831053931
OpenAlex ID: W7154448663