Work

Technical Bulletin for GROQ-seq TEV Protease Function Assay

Year: 2026

Type: article

Abstract: This technical bulletin presents a pooled assay for measuring tobacco etch virus (TEV) protease function using the GROQ-seq platform and its first generated dataset. The assay links enzyme function to cellular fitness via antibiotic resistance, enabling high-throughput functional profiling by sequencing. The resulting GROQ-seq TEV Protease assay produces calibrated function measurements spanning an approximate 100-fold dynamic range. Its first generated dataset includes over 18,000 TEV protease variants across 24 different experimental conditions. This work and resulting dataset support the development of predictive models for assessing mutational effects.

Source: Zenodo (CERN European Organization for Nuclear Research)

DOI: 10.5281/zenodo.19551806

Authors: Cortade, Dana, McLellan, James, Baranowski, Catherine, Apel, Amanda, Kelly, Peter J., Spinner, Aviv, Sisson, Zach, Dhroso, Andi, Hudson, Corey M., Chadha, Anjali, DeBenedictis, Erika, Ikonomova, Svetlana, Ross, David

Institutions: The Align Foundation, Sandia National Laboratories California, National Institute of Standards and Technology

Language: English

Topic: CRISPR and Genetic Engineering

Subfield: Molecular Biology

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

Open Access status: green

OpenAlex ID: W7154497654

FWCI: 4.159

Cites:

Cited by: 2

Related to: 10